The average spacing of restriction enzyme recognition sites in DNA

The average spacing of restriction enzyme recognition sites in DNA.

The discovery of naturally occurring enzymes which cleave DNA at sites specific to particular nucleotide sequences has had a great impact on molecular biology. The function of these enzymes in uivo is to protect bacterial cells from viral invasion by degradation of foreign DNA. Several hundred of these “restriction” enzymes are known and they are a very common tool both for analysis and manipul...

Restriction Enzyme Recognition Sequence

A critical and difficult part of characterizing restriction enzymes and methylases is the identification of recognition sequences. To simplify this process, we have developed a plasmid transformation method along with a computer program named RM search that determines the exact recognition sequences for given restriction and modifica-

Characterization of Avian Pasteurella multocida Isolates by Protein Profiles and Restriction Enzyme Analysis of Chromosomal DNA

Sequence determination of restriction-endonuclease recognition sites.

the enzyme DpnII, produced by another strain of D. pneumoniae, recognizes the same sequence, but is inhibited by this same methylated base. The significance of the occurrence of modified nucleotides in eukaryotic DNA is not as yet understood, although a relationship between modification and gene expression has been proposed by several workers (Waalwijk & Flavell, 1978; Bird et al., 1979). By us...

Reliable detection of DNA cytosine methylation at CpNpG sites using the engineered restriction enzyme EcoRII-C.

Methylation of cytosine to 5-methylcytosine (mC) is the most important epigenetic DNA alteration in eukaryotes. Cytosine methylation is involved in establishing a silenced chromatin stage through interaction with DNA-binding proteins and recruitment of histone deacetylases and other histone-modifying enzymes leading to chromatin remodeling. In this fashion, DNA methylation is connected with pro...